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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
31/03/2011 |
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Data da última atualização: |
23/02/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SANTOS, A. F. dos; TESSMANN, D. J.; ALVES, T. C. A.; VIDA, J. B.; HARAKAWA, R. |
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Afiliação: |
ALVARO FIGUEREDO DOS SANTOS, CNPF; DAURI JOSÉ TESSMANN, Universidade Estadual de Maringá; TATIANE CRISTINA ALBUQUERQUE ALVES, Universidade Estadual de Maringá; JOÃO BATISTA VIDA, Universidade Estadual de Maringá; RICARDO HARAKAWA, Instituto Biológico. |
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Título: |
Root and crown rot of brazilian pine (Araucaria angustifolia) caused by Phytophthora cinnamomi. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Journal of Phytopathology, v. 159, n. 3, p. 194-196, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
In an area reforested with Brazilian pine (Araucaria angustifolia) located in Parana´ State, southern Brazil, 20- to 40-year-old trees representing 0.2% of the surveyed area had symptoms of root and crown rot, yellowing and browning of leaves from the uppermost branches and death. Three Phytophthora isolates obtained from diseased plant tissue were tested against 1-year-old Brazilian pine seedlings and found to display positive pathogenicity. Based on their morphological and physiological characteristics, the isolates were identified as Phytophthora cinnamomi. A GenBank BLAST search of partial sequences from the b-tubulin and elongation factor-1a genes, as well as the ITS regions and 5.8S gene of rDNA, confirmed the species identification. This is the first report of the involvement of this pathogen on the aetiology of Brazilian pine root and crown rot. |
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Palavras-Chave: |
Patologia florestal. |
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Thesagro: |
Fungo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01449naa a2200193 a 4500
001 1884177
005 2015-02-23
008 2011 bl uuuu u00u1 u #d
100 1 $aSANTOS, A. F. dos
245 $aRoot and crown rot of brazilian pine (Araucaria angustifolia) caused by Phytophthora cinnamomi.$h[electronic resource]
260 $c2011
520 $aIn an area reforested with Brazilian pine (Araucaria angustifolia) located in Parana´ State, southern Brazil, 20- to 40-year-old trees representing 0.2% of the surveyed area had symptoms of root and crown rot, yellowing and browning of leaves from the uppermost branches and death. Three Phytophthora isolates obtained from diseased plant tissue were tested against 1-year-old Brazilian pine seedlings and found to display positive pathogenicity. Based on their morphological and physiological characteristics, the isolates were identified as Phytophthora cinnamomi. A GenBank BLAST search of partial sequences from the b-tubulin and elongation factor-1a genes, as well as the ITS regions and 5.8S gene of rDNA, confirmed the species identification. This is the first report of the involvement of this pathogen on the aetiology of Brazilian pine root and crown rot.
650 $aFungo
653 $aPatologia florestal
700 1 $aTESSMANN, D. J.
700 1 $aALVES, T. C. A.
700 1 $aVIDA, J. B.
700 1 $aHARAKAWA, R.
773 $tJournal of Phytopathology$gv. 159, n. 3, p. 194-196, 2011.
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Registro original: |
Embrapa Florestas (CNPF) |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
25/07/2022 |
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Data da última atualização: |
25/08/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
OKINO, C. H.; MALAGO JUNIOR, W.; MARCONDES, C. R.; GIGLIOTI, R.; MONTASSIER, H. J.; OLIVEIRA, H. N. DE; OLIVEIRA, M. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; WILSON MALAGO JUNIOR, CPPSE; CINTIA RIGHETTI MARCONDES, CPPSE; RODRIGO GIGLIOTI, Centro de Pesquisa e Desenvolvimento de Genética e Biotecnologia, Instituto de Zootecnia (IZ), Nova Odessa; HÉLIO JOSÉ MONTASSIER, UNESP; HENRIQUE NUNES DE OLIVEIRA, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE. |
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Título: |
CD4 bovine gene: differential polymorphisms among cattle breeds and a new tool for rapid identification. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Veterinary Immunology and Immunopathology, v. 251, sep. 2022, 110462. |
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Páginas: |
18 p. |
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DOI: |
ttps://doi.org/10.1016/j.vetimm.2022.110462 |
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Idioma: |
Inglês |
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Conteúdo: |
Two mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals. |
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Palavras-Chave: |
High Resolution Melt; Immunophenotyping; QPCR; T helper cells. |
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Thesaurus NAL: |
Genotype. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/237927/1/CD4-Bovine-Gene.pdf
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Marc: |
LEADER 01840naa a2200277 a 4500
001 2144902
005 2022-08-25
008 2022 bl uuuu u00u1 u #d
024 7 $attps://doi.org/10.1016/j.vetimm.2022.110462$2DOI
100 1 $aOKINO, C. H.
245 $aCD4 bovine gene$bdifferential polymorphisms among cattle breeds and a new tool for rapid identification.$h[electronic resource]
260 $c2022
300 $a18 p.
520 $aTwo mutations in the CD4 bovine gene (G>T/Q306H; A>C/K310N) were identified as causative for altered staining with anti-CD4 mAb #CC8. We developed a HRM qPCR for genotyping these mutations and compare with immunophenotyping in different cattle breeds. The assay distinguished five genotypes, B (homozygous, G/A) and C (heterozygous, G/A and T/C), found in taurine, A (homozygous, G/C) and D (heterozygous, T/C and G/C), found in zebu. The E genotype (homozygous, T/C) was not observed in tested animals. As expected, B and C presented high/very high and intermediate CD4 staining, respectively. The lack/low CD4 staining was mainly related to the A, while the intermediate staining was mainly related to D genotype. The developed HRM qPCR assay accurately identified the altered phenotypes associated with CC8 staining in taurine. However, the assay cannot be applicable in zebu or hybrid breeds, probably due to additional mutations in the CD4 gene from zebu descendant animals.
650 $aGenotype
653 $aHigh Resolution Melt
653 $aImmunophenotyping
653 $aQPCR
653 $aT helper cells
700 1 $aMALAGO JUNIOR, W.
700 1 $aMARCONDES, C. R.
700 1 $aGIGLIOTI, R.
700 1 $aMONTASSIER, H. J.
700 1 $aOLIVEIRA, H. N. DE
700 1 $aOLIVEIRA, M. C. de S.
773 $tVeterinary Immunology and Immunopathology$gv. 251, sep. 2022, 110462.
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Embrapa Pecuária Sudeste (CPPSE) |
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